Figure 8.

No detectable contribution of non-β precursors to the maintenance of mature β-cells in adult mice. A: Graphical hypothesis illustrating two possible outcomes for the pulse-chase experiment with a focus on the changes in overall (mature) β-cells. In scenario 1, non-β progenitors contribute to the total pool of β-cells, leading to a gradual increase in the fraction of lineage-negative mature β-cells over time. In scenario 2, non-β progenitors do not contribute to the total pool of β-cells and thus the fraction of lineage-negative mature β-cells remain the same. B: Quantification of the percentage of lineage-positive mature β-cells among pulse and chase cohorts (n ≥ 3 mice per group). C: Distribution of lineage-positive mature β-cells from each individual mouse in the pulse and chase cohorts (n ≥ 3 mice per group, with at least 15 islets and a total of >20 virgin and 800 mature β-cells counted per animal). Open circles, females; closed circles, males. Data were analyzed for statistical significance by Kruskal-Wallis test, corrected for multiple comparisons with the Dunn test. Error bars represent ±SD. ns, not significant.