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. 2021 Jun 2;52:78. doi: 10.1186/s13567-021-00947-5

Figure 2.

Figure 2

Three-dimensional cell culture of mammary epithelial cells with Matrigel® gel. A Light microscopy images of mammosphere formation by cell self-assembly (BME-UV1 cell line, ×10 magnification) from the day of seeding to day 9 of culture. B Confocal microscopy images of a section of mammosphere in visible light (left) and following indirect immunofluorescence with anti-E-cadherin (CDH1, orthogonal view in the right panel) antibodies and Propidium iodide co-labeling (nuclei staining, CDH1/IP). Scale bars = 30 µM.