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. Author manuscript; available in PMC: 2021 Jun 3.
Published in final edited form as: Science. 2021 Apr 22;372(6540):371–378. doi: 10.1126/science.aba5545

Fig. 4. Epigenome affects require DNA replication and occur during the first S phase after RIF1 degradation.

Fig. 4.

(A) Average log2(observed/expected) within top 10% A- (top panel) and B- (bottom panel) compartment in control (black line) and RIF1 KD (gray line) cells at G1, early S, middle S, and late S/G2 time points. (B) Interaction frequency differences between control and RIF1 KD at top 10% EtL and LtE regions at G1, early S, middle S and late S/G2 timepoints whose RT is indicated by average high resolution Repli-Seq heatmaps. (C) H3K27ac ChIP-seq of Chr7 128–131.9 Mb in control (top), RIF1 KD (middle), and domainogram (bottom) indicating -log2 (Benjamini-Hochberg adjusted p-values) calculated for the differences of control subtracted from KD tracks (Methods) at G1, early S, middle S, and late S/G2 time points. (D) Heatmap showing log2(KD/control) of H3K27ac peaks at G1, early S, middle S, and late S/G2 time points. (E) H3K9me3 ChIP-seq of Chr10 64.05–67.6Mb in control (top), RIF1 KD (middle), and domainogram (bottom) at G1, early S, middle S, and late S/G2 time points. (F) Heatmap showing log2(KD/control) of H3K9me3 peaks at affected regions at G1, early S, middle S, and late S/G2 time points. (G) H3K9me3 ChIP-seq of Chr1 4.05–5.0 Mb in control (top), RIF1 KD (middle), and domainogram (bottom) at G1, early S, middle S, and late S/G2 time points. (H) Heatmap showing log2(KD/control) of H3K9me3 domains at unaffected regions at G1, early S, middle S, and late S/G2 time points.