Table 3.
Chlorophyll content in seedlings after the second chilling stress
| NA-Ye | CA-Ye | NA-MP | CA-MP | NA-DP | CA-DP | ||
|---|---|---|---|---|---|---|---|
| Chlorophyll a (μg ml-1FW) | BCS | 20.35 ± 6.52 | 19.27 ± 0.86 | 25.31 ± 8.78 | 15.53 ± 1.77 | 11.06 ± 0.71 | 11.47 ± 8.01 |
| ACS | 18.85 ± 2.01 | 22.12 ± 2.74 | 17.76 ± 7.75 | 22.5 ± 4.63 | 26.97 ± 6.63 | 17.3 ± 5.27 | |
| Chlorophyll b (μg ml-1FW) | BCS | 8.83 ± 3.27 | 6.03 ± 1.78 | 8.55 ± 3.61 | 6.82 ± 1.84 | 0.71 ± 0.99 | 1.71 ± 4.49 |
| ACS | 6.68 ± 4.77 | 9.79 ± 2.17 | 8.53 ± 6.21 | 8.16 ± 0.85 | 4.3 ± 3.28 | 3.93 ± 1.25 | |
| Total chlorophyll (μg ml-1FW) | BCS | 29.17 ± 9.79 | 25.3 ± 2.58 | 33.86 ± 12.36 | 22.35 ± 1.34 | 11.77 ± 0.31 | 13.18 ± 12.15 |
| ACS | 25.53 ± 6.78 | 31.91 ± 4.42 | 26.29 ± 13.87 | 30.66 ± 4.52 | 31.26 ± 5.98 | 21.23 ± 6.00 | |
| Chlorophyll a /chlorophyll b | BCS | 2.55 ± 0.27 | 4.01 ± 1.18 | 4.03 ± 1.39 | 2.64 ± 0.79 | 8.52 ± 7.65 | 1.93 ± 2.61 |
| ACS | 5.8 ± 5.05 | 2.52 ± 0.48 | 3.54 ± 2.84 | 5.62 ± 5.85 | 3.93 ± 4.11 | 5.15 ± 1.58 |
Effect of the second chilling stress by measuring chlorophyll content in seedlings of yellow- (Ye-), medium purple- (MP-), and deep purple (DP-) seed seedlings. Chlorophyll a, chlorophyll b, total chlorophyll, and chlorophyll a/chlorophyll b ratio, samples were taken before chilling stress (BCS) (18 °C) and after chilling stress (ACS) (4 °C for 6 h) under non-acclimated (NA) (18 °C) and chilling-acclimated (CA) (4 °C) conditions. Data are means ± SD of three biological replicates. Significant differences, evaluated by t-test, are marked by asterisks: *p ≤ 0.05, when compared to NA-BCS vs. CA-BCS and NA-ACS vs. CA-ACS within seed color group