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. 2021 Jun 3;19:81. doi: 10.1186/s12958-021-00753-w

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — The expression levels of let-7a, YAP1 and TAZ in JZ SMCs in the ADS group and control group. a The mRNA expression level of let-7a determined by RT-qPCR and normalized against that of U6. b The mRNA expression level of YAP1 determined by RT-qPCR and normalized against that of β-actin. c The mRNA expression level of TAZ determined by RT-qPCR and normalized against that of β-actin. d The protein expression levels of YAP1 and TAZ determined by western blotting and normalized against that of α-tubulin. e The relative fold protein expression of YAP1 normalized against α-tubulin. f The relative fold protein expression of TAZ normalized against α-tubulin. These experiments were performed two times with three replicates in each experiment. Significance was determined by Student’s t test; **p < 0.01, *p < 0.05