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. 2021 Jun 3;19:81. doi: 10.1186/s12958-021-00753-w

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Flow cytometry analysis of apoptosis and a CCK-8 assay performed with ADS JZ SMCs. a Cells were transfected with the let-7a overexpression lentiviral vector GV280 or lentiviral null vector for 72 h and then used for flow cytometry analysis. b The percentages of apoptotic cells in the lenti-GV280 group and lenti-NC group. c Verteporfin-treated cells were transfected with the let-7a overexpression lentiviral vector GV280 for 72 h and then used for flow cytometry analysis. d The percentages of apoptotic cells in the lenti-GV280 + verteporfin group and verteporfin group. e OD₄₅₀ values of the lenti-GV280 group and lenti-NC group. f OD₄₅₀ values of the lenti-GV280 + verteporfin group and verteporfin group. These experiments were performed two times with three replicates in each experiment. Significance was determined by Student’s t test; ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05