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. 2021 Jun 1;20:15330338211019442. doi: 10.1177/15330338211019442

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© The Author(s) 2021

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 3. — Signal intensity of PD-L1 in colon cancer tissues after sample deglycosylation. A, Representative images show the samples stained with programmed death ligand 1 (PD-L1) 28-8 monoclonal antibody (mAb), with or without deglycosylation. Bar = 500 µm (40x), Bar = 100 µm (200x). B, Signal intensity of PD-L1 stained by PD-L1 28-8 mAb, before and after deglycosylation. C, Doughnut chart represents the fold change (FC) of PD-L1 immunoreactivity score (IRS) after deglycosylation. Black: IRS repressed after tissue deglycosylation; Green: IRS increased ≤ 2 fold after tissue deglycosylation; Blue: IRS had no change after tissue deglycosylation; Red: IRS increased > 2 fold after tissue deglycosylation. D, Bar chart represents the signal intensity of PD-L1 stained with 28-8 mAb, before and after deglycosylation. Blue: Samples without deglycosylation; Green: Samples with deglycosylation. P < 0.001. All data are performed by GraphPad Prism 8.0.