Figure 3.

FEZ1-deficient neurons display collapsed growth cones. A–C, Immunostaining against CRMP1, FEZ1, and Tau in hippocampal neurons treated with FEZ1 sgRNA, LUC sgRNA, or uninfected controls, respectively, 6 DPI. Majority of the FEZ1-deficient neurons displayed smaller growth cones compared with the controls (red arrowheads). Scale bars: 20 μm. D, Quantification of growth cone sizes in FEZ1-deficient or control LUC sgRNA and uninfected neurons. On average, growth cones in control neurons were ∼4- to 5-fold larger than those in FEZ1-deficient neurons. Statistical significance was determined using Kruskal–Wallis analysis. FEZ1 sgRNA n = 30, LUC sgRNA n = 31, uninfected n = 33, collected over three independent experiments. All error bars represent SEM. Western blottings for CRIPSR/Cas9-mediated FEZ1 knock-down in neurons are shown in Extended Data Figure 3-1.