Table 1.
Antibodies used for immunoblotting and immunofluorescence in this study
| Dilution | Source | RRID | ||
|---|---|---|---|---|
| WB | IF | |||
| Primary antibody | ||||
| Mouse α-FLAG M2 | 1:1000 | - | Sigma-Aldrich | AB_262044 |
| Mouse α-CRMP1 | 1:1000 | 1:200 | Santa Cruz Biotechnology | AB_10846086 |
| Mouse α-VAMP2 | - | 1:200 | Synaptic Systems | AB_887811 |
| Rabbit α-βactin | 1:1000 | - | Synaptic Systems | AB_11042458 |
| Rabbit α-FEZ1 | 1:1000 | 1:400 | In house | |
| Rabbit α-GFP | 1:20,000 | - | Synaptic Systems | AB_887725 |
| Mouse α-MAP2 | - | 1:1000 | Merck | AB_477256 |
| Rabbit α-MAP2 | - | 1:1000 | Synaptic Systems | AB_2147096 |
| Mouse α-Myc | 1:1000 | - | In house | |
| Guinea Pig α-Tau | - | 1:400 | Synaptic Systems | AB_1547385 |
| Secondary antibody | ||||
| Goat α-mouse IgG (H + L)-HRP conjugate | 1:4000 | - | Bio-Rad | AB_11125547 |
| Goat α-rabbit IgG (H + L)-HRP conjugate | 1:4000 | - | AB_11125142 | |
| Donkey α-mouse IgG (Cy2) | - | 1:200 | Jackson ImmunoResearch | AB_2340827 |
| Donkey α-mouse IgG (Cy3) | - | 1:200 | AB_2315777 | |
| Donkey α-rabbit IgG (Cy2) | - | 1:400 | AB_2340612 | |
| Donkey α-rabbit IgG (Cy3) | - | 1:200 | AB_2307443 | |
| Donkey α-guinea pig IgG (Cy5) | - | 1:400 | AB_2340462 | |