Table 2.
Quality control parameters of comprehensive transcriptome sequencing (RNA-Seq).
Parameters | (N) Thp-1 mock | (O) Thp-1 + N6 wt MOI10 | (Q) Thp-1 + N6 858 MOI10 | (Z) Thp-1 mock | (A1) Thp-1 + ADP-heptose 5 µM | (A2) Thp-1 mock | (B2) Thp-1 + ADP-heptose 5 µM | |
---|---|---|---|---|---|---|---|---|
Mapping statistics | Reads mapped (%) | 81.85 | 81.88 | 80.87 | 83.99 | 82.23 | 82.55 | 82.03 |
Reads not mapped (%) | 18.15 | 18.12 | 19.13 | 16.01 | 17.77 | 17.45 | 17.97 | |
Fragment counting | Unique (%) | 79.12 | 79.25 | 78.1 | 81.47 | 79.52 | 79.83 | 79.27 |
Non-specifically (%) | 2.73 | 2.61 | 2.77 | 2.52 | 2.71 | 2.72 | 2.76 | |
Counted fragments by type | Intergenic (%) | 11.17 | 10.79 | 11.65 | 9.56 | 10.26 | 7.72 | 8.03 |