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. 2021 Mar 5;10(6):e018097. doi: 10.1161/JAHA.120.018097

Figure 3. AdipoRon attenuates cardiopulmonary bypass (CPB)–induced myocardial inflammation.

Figure 3

Myocardial mRNA expression of (A) the cytokines/chemokines TNF‐α (tumor necrosis factor α), IL (interleukin) 1β, CCL2 (C‐C chemokine ligand 2), and IL‐10, as well as (B) the enzymes nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (Nox2 subunit) and inducible nitric oxide synthase (iNOS) was measured relative to HPRT1 (hypoxanthine phosphoribosyltransferase 1) by quantitative real‐time polymerase chain reaction in AdipoRon‐ and vehicle‐treated animals after completion of the experimental CPB procedure. C, Expression of nuclear factor κB inhibitor α (IκBα) in myocardial tissue of AdipoRon‐ and vehicle‐treated rats was analyzed by immunoblot. Left panel: representative pictures of the IκBα (molecular weight: 39 kDa) and GAPDH, molecular weight: 37 kDa) band patterns. Right panel: column bars indicate quantified levels of IκBα/GAPDH expression ratios. Results are presented as mean+SEM (n=7 animals per group). Differences of marker expression levels between experimental groups were analyzed statistically by performing the indicated pairwise comparisons.