Fig. 6.

Over-expression of PPARα restores Nurr1-upregulating efficacy of gemfibrozil in PPARα^−/− DA neurons. Primary DA neurons harvested from PPARα^−/− mice were transduced with lenti-empty vector e-GFP (a, b), lenti-full-length PPARα (c, d), or lenti-Y464D PPARα (e, f) for 24 h. Then cells were treated with 10 μM of gemfibrozil for 18 h followed by immunolabeling for Nurr1 (Cy5; red). MFI of Nurr1-ir was quantified for an average of 800 cells per group (b lenti-eGFP; d lenti-FL PPARα; f lenti-Y464D PPARα). A paired t test was utilized to compare significant differences from the means of these two groups resulting in t = 0.4487₁₀; p = 0.66 (b). An unpaired t test analysis was performed to show significant differences between the means (t = 11.81₁₀; = p < 0.0003) (d). Unpaired t test shows t = 0.29₁₀; p = 0.77 (f). All results are mean ± SD of three independent experiments