Fig. 7.

Oral administration of gemfibrozil increases Nurr1 expression in vivo in nigra via PPARα. WT (a, b) and PPARα^−/− (c, d) animals were gavage fed 7.5 mg/kg gemfibrozil or 0.5% methyl cellulose (Veh) for 14 days via oral route. After that, nigral sections were double-labeled with anti-mouse TH (cy2) and anti-rabbit Nurr1 (cy5) antibodies. Total n = 5 animals were used per group. e MFI analysis of nigral sections indicates a significant difference between genotypes [(F_(3,23) = 233.55> F_c = 3.287); *** = p < 0.0003]. f Representative immunoblots using isolated nigral sections from both WT and PPARα^−/− animals in this experimental paradigm. g Densitometric analysis of f indicates a significant increase in Nurr1 protein expression from WT, but not PPARα^−/− animals fed GEM as shown by a two-way ANOVA [(F_{(3, 11)} = 20.01 > F_c = 4.757); ***p < 0.0003]