| Cell line (Mus musculus) |
J774A.1 |
PMID:1612739
|
|
Immortalized murine macrophages |
| Cell line (Mus musculus) |
TIB73 |
ATCC: BNL CL.2 |
|
Immortalized murine hepatocytes |
| Strain, strain background (Mus
musculus) |
C57BL/6J |
Jackson Laboratories: 000664 |
|
|
| Strain, strain background (Mus
musculus) |
gp91phox- (C57BL/6J backcross) |
Jackson Laboratories: 002365 |
|
|
| Strain, strain background (Escherichia
coli) |
DH10b |
Invitrogen: 10536193 |
|
|
| Strain, strain background (Escherichia
coli) |
SM10 |
DOI: https://doi.org/10.1038/nbt1183-784
|
|
|
| Strain, strain background (Escherichia
coli) |
BL21 (DE3) |
EMD Millipore: 69450 |
|
|
| Strain, strain background (Escherichia
coli) |
Rosetta (DE3) |
EMD Millipore: 70954 |
|
|
| Strain, strain background (Listeria
monocytogenes) |
10403S |
PMID:2125302
|
WT |
|
| Strain, strain background (Bacillus
subtilis) |
168 |
PMID:18723616
|
WT |
|
| Strain, strain background (Francisella
novicida) |
U112 |
PMID:17550600
|
WT |
|
| Strain, strain background (Pseudomonas
aeruginosa) |
PAO1 |
PMID:20023018
|
WT |
|
| Strain, strain background (Staphylococcus
aureus) |
Newman |
PMID:17951380
|
WT |
|
| Strain, strain background (Enterococcus
faecalis) |
OG1RF |
PMID:18611278
|
WT |
|
| Strain, strain background (Listeria
monocytogenes) |
DP-L3903 |
PMID:11500481
|
Antibiotic marked competition strain |
|
| Strain, strain background (Listeria
monocytogenes) |
DP-L4056 |
PMID:12107135
|
Phage-cured 10403S strain for use with pPL1 integration
plasmid |
|
| Genetic reagent |
Genetically modified bacterial strains used in this
work |
|
This paper |
Supplementary file 1 |
| recombinant DNA reagent |
pPL1077 |
This study |
pPL1-GFP integration plasmid |
(Gift from Peter Lauer; Berkeley, CA) |
| Recombinant DNA reagent |
pKSV7 |
PMID:8388529
|
Shuttle vector for L. monocytogenes
gene disruption |
|
| Recombinant DNA reagent |
pET20b |
Novagen: 69739 |
C-terminus 6xHis tagged E. coli T7
expression vector |
|
| Recombinant DNA reagent |
pET28b |
Novagen: 69865 |
N-terminus 6xHis tagged E. coli T7
expression vector |
|
| Recombinant DNA reagent |
pPL2-Pspac |
PMID:25583510
|
Integrative L. monocytogenes plasmid
pPL2 engineered with constitutive Pspac promoter |
|
| Recombinant DNA reagent |
pHT01 |
PMID:17624574
|
B. subtilis expression vector |
|
| Recombinant DNA reagents |
Plasmids generated and used in this work |
|
This paper |
Supplementary file 2 |
| Gene (Listeria monocytogenes) |
lmo0103
(LMRG_02352) |
GenBank: Gene ID 12552319 |
Gene rha1
|
|
| Gene (Listeria monocytogenes) |
lmo0613 (LMRG_00296) |
GenBank: Gene ID 12552833 |
Gene rha2
|
|
| Gene (Homo sapiens) |
akr1c1 |
GenBank: Gene ID 1645 |
Gene akr1c1
|
|
| Gene (Arabidopsis thaliana) |
p1-zcr |
GenBank: Gene ID 831560 |
Gene p1-zcr
|
|
| Sequence-based reagents |
RT-qPCR primers |
This paper |
|
(See supplementary file 3) |
| Sequence-based reagents |
Cloning primers |
This paper |
|
(See supplementary file 3) |
| Sequence-based reagents |
Gene coding sequences for protein expression |
This paper |
|
(See supplementary file 4) |
| Chemical, compound, drug |
4-HNE |
Cayman Chemical: 32100 |
64 mM 4-HNE in absolute ethanol |
|
| Antibody |
Anti-4-HNE adduct |
Abcam: ab46545 |
Rabbit IgG polyclonal antibody |
(1:200) |
| Antibody |
Anti-actin |
Abcam: ab8226 |
Mouse IgG monoclonal antibody |
(1:1000) |
| Antibody |
Anti-rabbit secondary antibody |
Licor: 926–32211 |
Goat IgG monoclonal antibody |
(1:8000) |
| Antibody |
Anti-mouse secondary antibody |
Licor: 926–68072 |
Donkey IgG monoclonal antibody |
(1:8000) |
| Antibody |
Anti-GFP primary antibody |
Invitrogen: MA5-15256 |
Mouse IgG monoclonal antibody |
(1:500) |
| Antibody |
Isotype control primary antibody, histology |
R and D Systems: AB-105-C |
Normal Rabbit IgG |
(1:1000) |
| Commercial assay, kit |
Ovation Complete Prokaryotic RNA-Seq System |
Nugen: 0363–32 |
RNA-seq processing kit |
|
| Software, algorithm |
Rockhopper |
cs.wellesley.edu/~btjaden/Rockhopper/ |
RNA-seq data analysis software |
|
