Table 2.
Real-time PCR primer sequences.
| Target gene | Primer sequence forward (5′ → 3′) | Primer sequence reverse (5′ → 3′) | Temperature (°C) | Accession number |
|---|---|---|---|---|
| IL-6 | CAGCAGAATGGGGGAGTTATC | CTCGCAGAGTCTTGACATCCTT | 62.3 | KC535507.1 |
| IL-12p35 | TGGAAAAGGAGGGGAAGATG | AGACGGACGCTGTGTGAGTGTA | 55.4 | KF944667.1 |
| IL-12p40 | ACAAAGATGAAAAACTGGAGGC | GTGTGTGGTTTAGGTAGGAGCC | 59 | KF944668.1 |
| IL-15 | CCTTCCAACAATCTCGCTTC | AACACATCTTCCAGTTCTCCTT | 61.4 | KT445872.1 |
| IL-17D | GTGTCCAGGAGAGCACCAAG | GCGAGAGGCTGAGGAAGTT T | 62.3 | KF245426.1 |
| IL-4/13A | CTACTGCTCGCTTTCGCTGT | CCCAGTTTTCAGTTCTCTCAGG | 55.9 | KT445871.1 |
| IL-4/13B | TGTGAACCAGACCCTACATAACC | TTCAGGACCTTTGCTGCTTG | 55.9 | KT625600.1 |
| TNF-α | CGCTGCTGTCTGCTTCAC | CCTGGTCCTGGTTCACTC | 58.4 | HQ696609 |
| IFN-γ2 | TGTTTGATGACTTTGGGATG | TCAGGACCCGCAGGAAGAC | 60.4 | JX657682 |
| IL-1β | AGAGTTTGGTGAAGAAGAGG | TTATTGTGGTTACGCTGGA | 57.1 | JQ692172 |
| TGF-β1 | TTGGGACTTGTGCTCTAT | AGTTCTGCTGGGATGTTT | 55.9 | EU099588 |
| TGF-β2 | TACATTGACAGCAAGGTGGTG | TCTTGTTGGGGATGATGTAGTT | 55.9 | KM279716 |
| IL-10 | AATCCCTTTGATTTTGCC | GTGCCTTATCCTACAGTATGTG | 61.4 | HQ388294 |
| IL-11 | GGTTCAAGTCTCTTCCAGCGAT | TGCGTGTTATTTTGTTCAGCCA | 57 | KT445870.1 |
| Hepcidin | AGCAGGAGCAGGATGAGC | GCCAGGGGATTTGTTTGT | 59.3 | JQ246442.1 |
| LEAP-2A | TGCCTACTGCCAGAACCA | AATCGGTTGGCTGTAGGA | 59.3 | FJ390414 |
| LEAP-2B | TGTGCCATTAGCGACTTCTGAG | ATGATTCGCCACAAAGGGG | 59.3 | KT625603.1 |
| βdefensin-1 | TTGCTTGTCCTTGCCGTCT | AATCCTTTGCCACAGCCTAA | 58.4 | KT445868.1 |
| Mucin2 | GAGTTCCCAACCCAACACAT | AAAGGTCTACACAATCTGCCC | 60.4 | KT625602 |
| CTL1 | GAACCGCAGGAAGTCCAGTG | GCTGACAGGCGAGGATGAACT | 60.7 | MN904650 |
| CTL2 | AACTTCGTGACAGCATTGGG | ATGGCAAGAATGAGGGAACC | 58.6 | MN904651 |
| CTL4 | GGTCATTGCGATGGTGGTC | CAGATACCGAAGGCTCCGAC | 59.2 | MN904652 |
| CTL5 | GCAAAGGAAATCGGCATC | GCGGTGAACCTCAGCAGC | 57.8 | MN904653 |
| CHT1 | TCCTCATCACCCACACGA | CCGACTCCTCCATCCTCTC | 55.4 | MN904654 |
| NF-κB p52 | TCAGTGTAACGACAACGGGAT | ATACTTCAGCCACACCTCTCTTAG | 58.4 | KM79720 |
| NF-κB p65 | GAAGAAGGATGTGGGAGATG | TGTTGTCGTAGATGGGCTGAG | 62.3 | KJ526214 |
| c-Rel | GCGTCTATGCTTCCAGATTTACC | ACTGCCACTGTTCTTGTTCACC | 59.3 | KT445865 |
| IκBα | TCTTGCCATTATTCACGAGG | TGTTACCACAGTCATCCACCA | 62.3 | KJ125069 |
| IKKα | GGCTACGCCAAAGACCTG | CGGACCTCGCCATTCATA | 60.3 | KM279718 |
| IKKβ | GTGGCGGTGGATTATTGG | GCACGGGTTGCCAGTTTG | 60.3 | KP125491 |
| IKKγ | AGAGGCTCGTCATAGTGG | CTGTGATTGGCTTGCTTT | 58.4 | KM079079 |
| JAK1 | TTTGCTGCACTGGTGGACA | GCGCAGGACATAGGTTCCTT | 60.0 | KT724352.1 |
| JAK2 | AGAGGCCATCGAGAGCTACT | TCATACGCCCCAACTGCAA | 59.7 | JF825474.1 |
| JAK3 | GCCGTTCAAGTGTCTGGAGA | AACTCAGCCTCCATGCACT | 59.5 | KU200686.1 |
| TYK2 | TTCGCCGTGTGTTTGCAAA | ACGCCAAAATGAGGAGCCA | 59.7 | KT724353.1 |
| STAT3a | ACATTCCTGCTGCGCTTCA | ACGAGGATGTTGGTGGCAT | 59.8 | KC978890 |
| STAT3b1 | TCAACATGGCCCAGTGGAA | AGCGTTGCGTGAGATTCCT | 59.4 | KU559609 |
| STAT3b2 | GCTGACCAACCATCCAAA | CGGAGTAGTTTACACACGGAC | 54.5 | KU559610 |
| β-Actin | GGCTGTGCTGTCCCTGTA | GGGCATAACCCTCGTAGAT | 61.4 | M25013 |