Skip to main content
. Author manuscript; available in PMC: 2021 Jun 3.
Published in final edited form as: FASEB J. 2020 Jan 9;34(2):3305–3317. doi: 10.1096/fj.201902915R

FIGURE 5.

FIGURE 5

Downregulation of N-WASP blocked P aeruginosa induced permeability in human microvascular endothelial and human alveolar epithelial cells. A and B, Western blot showed the lentivirus-mediated knockdown effects of N-WASP in human microvascular endothelial cells (HMVECs) in panel A and a human alveolar epithelial cell line (H441) in panel B by three different lentiviral shRNA constructs. The #2 lentiviral vector had the optimal efficiency and used for all following experiments. C and D, Measurement of monolayer resistance across HMVEC (C) and H441 monolayers (D) with or without N-WASP downregulation in response to P aeruginosa stimulation (MOI = 40). Electrical resistance was measured as described above up to 24 h post P aeruginosa stimulation. E and F, Resistance value was calculated based on panels C and D, in HMVEC cells (E) and H441 cells (F) with or without N-WASP downregulation. Data are presented as the percentage of decreased resistance normalized to control (n = 7–8). *P < .01, **P < .001