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. 2021 Jun 3;16(6):e0251920. doi: 10.1371/journal.pone.0251920

Fig 4. The relative expression of target sHSP20.0 normalized by the recommended set of references (RPL32 and TBP), the optimal reference (RPL32) and the least stable gene (GAPDH), respectively.

Fig 4

Columns represent the expressions of sHSP20.0 in Phaedon brassicae when subjected to cold shock (10°C), heat shock (40°C) and the control (25°C) for 2 h. Error bars indicate SE; *, P < 0.05; **, P < 0.01 (Student’s t-test by SAS 9.20).