Table 1. Primers for candidate reference genes used in RT-qPCR analyses.
| Genes | Accession No. | Primer sequence (5′-3′) | Product length (bp) | Primer efficiency (%E) | Regression coefficient (R2) | Linear regression |
|---|---|---|---|---|---|---|
| Actin1 | MW509776 | F: TTCCAATTGCTGGTCGAAAC | 204 | 98.75 | 0.9985 | y = -3.3522x+25.024 |
| R: AATTCGAGCCGTCGTACCTT | ||||||
| Actin2 | MW509777 | F: TGTCGTAGTGGATTCGGGAG | 125 | 102.24 | 0.999 | y = -3.2694x+26.274 |
| R: ACTTGATGAGGTACCGGGTG | ||||||
| EF-1α | MW509779 | F: TAGGTCGTGTGGAAACTGGTG | 167 | 96.76 | 0.9996 | y = -3.4021x+19.701 |
| R: TTCCTTGACGGAGACGTTCTT | ||||||
| GAPDH | MW509780 | F: CTCTTGTCGGCAAACTCACC | 194 | 104.50 | 0.9994 | y = -3.2185x+22.762 |
| R: GATGAAATCGGACGAGACGA | ||||||
| α-TUB | MW509778 | F: TGGACAGGATCAGGAAGCTC | 144 | 96.16 | 0.9991 | y = -3.4176x+20.745 |
| R: GCTTCGACTTCTTGCCGTAG | ||||||
| RPL19 | MW509781 | F: GCATTGTGGGTTTGGAAAGA | 157 | 103.12 | 0.9999 | y = -3.2493x+21.884 |
| R: CTTCATGTACAGGGCGTGGT | ||||||
| RPL32 | MW509782 | F: ACTGGCGTAAACCGAAAGGT | 178 | 99.03 | 0.9998 | y = -3.3455x+19.261 |
| R: CGGTTCTGCATGAGAAGGAC | ||||||
| TBP | MW509783 | F: GCAAGCAGCAAGAAGGTTTG | 207 | 106.75 | 0.9952 | y = -3.1701x+26.167 |
| R: GGGTGGCTTTTGGACTTTTC |