Table 2. Stability of reference gene expression under different experimental conditions calculated by four different analytical tools.

Experimental conditions	Reference genes	ΔCt		Bestkeeper		NormFinder		geNorm		Recommendation
		Stability	Rank	Stability	Rank	Stability	Rank	Stability	Rank
Development stage	Actin1	0.628	3	0.337	2	0.259	4	0.528	5	TBP
	Actin2	0.706	6	0.343	3	0.364	6	0.554	6
	EF-1α	0.641	4	0.230	1	0.306	5	0.420	3
	GAPDH	0.970	8	0.845	8	0.608	8	0.700	8
	α-TUB	0.837	7	0.469	6	0.511	7	0.611	7	RPL19
	RPL19	0.588	2	0.408	4	0.188	1	0.336	1
	RPL32	0.647	5	0.536	7	0.257	3	0.498	4
	TBP	0.585	1	0.432	5	0.191	2	0.336	1
Tissue	Actin1	1.157	7	0.567	5	0.744	7	0.694	5	EF-1α
	Actin2	1.134	6	0.456	4	0.723	6	0.860	6
	EF-1α	0.782	1	0.405	3	0.197	1	0.180	1
	GAPDH	1.204	8	1.091	8	0.795	8	1.006	8
	α-TUB	1.023	5	0.889	7	0.596	5	0.942	7	RPL32
	RPL19	0.930	3	0.091	1	0.348	3	0.600	4
	RPL32	0.863	2	0.356	2	0.260	2	0.458	3
	TBP	0.969	4	0.829	6	0.496	4	0.180	1
Temperature	Actin1	0.904	6	0.983	6	0.450	6	0.603	5	RPL32
	Actin2	0.882	4	0.988	7	0.424	5	0.576	4
	EF-1α	0.896	5	0.611	3	0.411	4	0.661	6
	GAPDH	1.398	8	1.575	8	0.894	8	0.922	8	TBP
	α-TUB	0.795	3	0.664	5	0.303	3	0.464	3
	RPL19	1.084	7	0.288	1	0.647	7	0.763	7
	RPL32	0.692	1	0.601	2	0.022	1	0.354	1
	TBP	0.722	2	0.655	4	0.137	2	0.354	1
Insecticide	Actin1	1.479	8	1.177	7	0.939	8	1.034	8	RPL19
	Actin2	0.996	5	0.812	6	0.445	4	0.767	6
	EF-1α	0.990	4	0.362	2	0.532	6	0.394	1
	GAPDH	1.154	7	1.181	8	0.615	7	0.886	7
	α-TUB	0.885	3	0.691	5	0.314	2	0.700	5	RPL32
	RPL19	0.876	1	0.482	3	0.359	3	0.394	1
	RPL32	0.884	2	0.535	4	0.281	1	0.655	4
	TBP	1.009	6	0.331	1	0.510	5	0.591	3