Fig 7. Latency establishment and reactivation of HTLV-1 does not depend on CTCF binding.

JET cells were infected by coculturing with lethally γ-irradiated 729B/HTLV-1, 729B/HTLV-1p12stop or 729B/HTLV-1 ΔCTCF and single infected cell clones were selected via limiting dilution. RFP (the indicator of Tax expression) positive or negative cells were examined and percentages of latent (RFP negative) and active (RFP positive) HTLV-1 infected cells are shown in panel A. RFP negative cells were activated with PMA/Ionomycin for 48hr. RFP positive and negative cells were counted and the result from three experiments is presented with standard errors in panel B.