Table 1. Summary of increased methylation in 5’LTR, env, pX region (sense) and 3’LTR/hbz (antisense) of HTLV-1ΔCTCF compared to wild type HTLV-1 or HTLV-1p12stop.
| PBMC |
Fold increase (≥ 2 fold) |
Methylated CpG sites (≥ 2 fold increase) vs total examined CpG sites (%) |
Methylation level at each CpG site (%) (Average % is shown in each parenthesis) |
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| HTLV-1 | ΔCTCF / WT |
ΔCTCF / p12stop |
ΔCTCF / WT |
ΔCTCF / p12stop |
WT | p12stop | ΔCTCF | |
| Sense Strand | 5’-LTR (166-679 | 2-15 | 2-15 | 15/40 (38%) | 16/40 (40%) | 0.2-1.5 (0.4%) | 0.2-5.2 (0.7%) | 0.1-5.3 (0.9%) |
| pX (7023-7719) | 2-120 | 2-43 | 19/20 (95%) | 13/20 (65%) | 0.4-25 (2.8%) | 0.9-31 (4.7%) | 2-90 (25.5%) | |
| Antisense Strand | 3’-LTR (8280-8959 | 2-6 | 2-7 | 14/48 (29%) | 15/48 (31%) | 0-0.35 (0.9%) | 0-11.7 (2.9%) | 0.1-9.5 (4.9%) |
| hbz (6647-8235) | 2-10 | 2-6 | 35/50 (70%) | 20/50 (40%) | 0-66.3 (6.4%) | 0-53.9 (7.5%) | 0-85.5 (12%) | |
| JET |
Fold increase (≥ 2 fold) |
Methylated CpG sites (≥ 2 fold increase) vs total examined CpG sites (%) |
Methylation level at each CpG site (%) (Average % is shown in each parenthesis) |
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| HTLV-1 | ΔCTCF / WT |
ΔCTCF / p12stop |
ΔCTCF / WT |
ΔCTCF / p12stop |
WT | p12stop | ΔCTCF | |
| Sense Strand | 5’-LTR (166-679) | 20-60 | 20-140 | 2/40 (5%) | 3/40 (7.5%) | 0.2-7.6 (0.7%) | 0.1-2.2 (0.4%) | 0.2-13.2 (0.8%) |
| pX (7023-7719) | 2-12 | 2-31 | 13/20 (65%) | 11/20 (55%) | 0-15.5 (2%) | 0.32-13.1 (1.7%) | 1-37 (6.6%) | |
| Antisense Strand | 3’-LTR (8280-8959) | 2-40 | 2-30 | 12/48 (25%) | 10/48 (21%) | 0-2.5 (1.1%) | 0-3 (0.9%) | 0-5 (1.9%) |
| hbz (6647-8235) | 2-5 | 2-5 | 11/50 (22%) | 20/50 (40%) | 0.2-20.9 (5.3%) | 0-20.3 (4%) | 0-14 (3.7%) | |