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. 2021 Mar 15;320(5):F719–F733. doi: 10.1152/ajprenal.00459.2020

Figure 1.

Figure 1.

Extracellular K+ rapidly dephosphorylates NaCl cotransporter (NCC) and STE20/SPS1-related proline-alanine-rich protein kinase (SPAK) in kidney slices ex vivo. A: protein extracts from 300-μM kidney slice homogenates that were incubated in 0 mM K+ for 1 h followed by incubations in 0 mM, 3 mM, 6 mM, or 10 mM K+ for 30 min were immunoblotted for phosphorylated (p)NCC at Thr53 (pNCC-T53), NCC, and β-actin. B: plot depicting the densitometric ratios of pNCC-T53 to NCC analyzed by ANOVA (F = 30.77, P < 0.0001); individual differences, by Tukey’s test, are shown. C: extracts blotted for pSPAK at Ser383 (pS383-SPAK), full-length SPAK (FL-SPAK), and β-actin. D: plot depicting the densitometric ratios of pSPAK to SPAK, analyzed by ANOVA (F = 25.30, P < 0.0001); individual differences, by Tukey’s test, are shown. Statistical analysis was performed on data from kidney slices from five different mouse kidneys. Each solid black circle represents protein extract from an individual mouse. NS, not significant; pOxSR1-S335, phosphorylated oxidative stress responsive kinase 1 at Ser335.