Table 1.
Antibodies
| Antibody | Species | Source and Validation | Dilution for Western Blot | Dilution for Immunofluorescence |
|---|---|---|---|---|
| tNCC | Rabbit | Ellison Laboratory (46) | 1:5,000 | |
| pNCC (T53) | Rabbit | Ellison Laboratory (25) | 1:2,000 | |
| SPAK | Rabbit | McCormick Laboratory (42) | 1:5,000 | 1:5,000 |
| OxSR1 | Rabbit | (6) | 1:5,000 | |
| pSPAK (S383) | Rabbit | Millipore, 07-2273 | 1:1,000 | 1:100 |
| β-Actin | Rabbit | Abcam, ab8227 | 1:5,000 | |
| Myc | Rabbit | Sigma-Aldrich, C3956 | 1:1,000 | |
| Calcineurin α | Mouse | Sigma-Aldrich, C1956 | 1:1,000 | |
| PP1α | Rabbit | Epitomics, 1950-1 | 1:1,000 | |
| Parvalbumin | Guinea pig | Swant, GP72 | 1:2,000 | |
| Calbindin | Mouse | Swant, calbindin D-28, 300 | 1:2,000 |
NCC, NaCl cotransporter; OxSR1, oxidative stress responsive kinase 1; PP1α, α subunit of protein phosphatase 1; pNCC (T53), phosphorylated NaCl cotransporter (recognizes threonine 53); pOxSR1, phosphorylated oxidative stress responsive kinase 1; pSPAK (S383), phosphorylated SPAK (recognizes serine 383); SPAK, STE20/SPS-1-related proline/alanine-rich kinase; tNCC, total NCC.