Figure 1.

Deletion of neural precursor cell expressed developmentally downregulated protein 4-2 (Nedd4-2) abolishes the effect of dietary Na⁺ intake on the basolateral K⁺ channel in the distal convoluted tubule (DCT). A and B: representative single channel recordings showing basolateral K⁺ channel activity in the DCT of wild-type [WT; floxed neural precursor cell expressed developmentally downregulated gene 4-like (Nedd4l^fl/fl)] mice (A) and kidney-specific Nedd2-4 knockout (Ks-Nedd4-2 KO) mice (B) on normal-salt (NS), high-salt (HS), or low-salt (LS) diets for 14 days, respectively. C: scatterplot summarizing the results of experiments in which Kir4.1/Kir5.1 channel activity in the DCT [defined by channel activity (NP_o)] was measured in both male and female WT and Ks-Nedd4-2 KO mice on different Na⁺ diets for 14 days. The experiments were performed in cell-attached patches at a holding potential of 0 mV. The DCT was bathed in a solution containing 140 mM NaCl/5 mM KCl, and the pipette solution contained 145 mM K⁺. Significance was determined by two-way ANOVA.