Figure 3.

Deletion of neural precursor cell expressed developmentally downregulated protein 4-2 (Nedd4-2) abolishes the effect of dietary Na⁺ intake in the distal convoluted tubule (DCT) membrane potential. A: perforated whole cell recordings showing K⁺ current (I_K) reversal potential in the DCT of wild-type [WT; floxed neural precursor cell expressed developmentally downregulated gene 4-like (Nedd4l^fl/fl)] mice on normal-salt (NS), high-salt (HS), and low-salt (LS) diets for 14 days, respectively. B: perforated whole cell recording showing the I_K reversal potential in the DCT of kidney-specific Nedd4-2 knockout (Ks-Nedd4-2 KO) mice on NS, HS, and LS diets for 14 days, respectively. (A trace from a control mouse on NS is included as a reference.) C: scatterplot summarizing the results of experiments in which the I_K reversal potential was measured in both male and female WT and Ks-Nedd4-2 KO mice on different Na⁺ diets for 14 days. Mean values and SEs are shown on the left of each column. For the measurement of I_K reversal potential, the bath solution contained 140 mM NaCl and 5 mM KCl and the pipette solution had 140 mM KCl. Significance was determined by two-way ANOVA.