Figure 5.

High salt (HS) inhibits epithelial Na⁺ channels (ENaC) in wild-type [WT; floxed neural precursor cell expressed developmentally downregulated gene 4-like (Nedd4l^fl/fl)] mice but not kidney-specific neural precursor cell expressed developmentally downregulated protein 4-2 (Nedd4-2) knockout (Ks-Nedd4-2 KO) mice. A: set of gap-free recordings showing amiloride-sensitive Na⁺ currents in the late distal convoluted tubule (DCT2)/connecting tubule (CNT) measured at −60 mV with the perforated whole cell patch in WT and Ks-Nedd4-2 KO mice on normal salt (NS) and HS (14 days; HS-14d), respectively. B: scatterplot summarizing the results of experiments in which amiloride (10 µM)-sensitive Na⁺ currents were measured at −60 mV with perforated whole cell recordings in the DCT2/CNT of male WT and Ks-Nedd4-2 KO mice on NS or HS diets for 14 days, respectively. Significance was determined by two-way ANOVA.