Figure 6.

Ca²⁺ and shrinkage responses to trypsin, tryptase, and 2-Furoyl-LIGRLO-NH₂ (2FLI) were eliminated in nasal turbinate serous acinar cells from protease-activated receptor 2 (PAR-2) knockout (par-2^−/−) mice. Representative traces showing Ca²⁺ (gray circles) and volume (open squares) responses in nasal turbinate cells from Wt mice C57Bl/6 mice stimulated with 1 µM trypsin (A), 1 µM tryptase (B), 10 µM 2FLI (C), 1 µM thrombin (A), heat-inactivated 1 µM tryptase (B), or 10 µM AY-NH₂ (C). Representative traces of cells from par-2^−/− mice during stimulation with trypsin (D), tryptase (E), 2FLI (F), or carbachol (CCh, D–F). Results are representative of ≥3 experiments using cells from three separate mice of each genotype.