Figure 5.

In vitro cell viability studies in BRAF V600E expressing cell lines (human A375 and murine YUMM1.G1 melanoma) and wild-type BRAF expressing melanoma cell line (murine B16-F10) after incubation with free vemurafenib, PLL-CNC NPs, and PLL-CNC V NPs at vemurafenib concentrations of 0.1, 0.5, 1.0, and 1.5 μM with respective CNC concentrations of 0.5, 2.2, 4.5, and 6.7 μg/mL for 6, 24, 48, and 72 h. For B16-F10 the assay was carried out only at 24 and 72 h. The negative (−) and positive (+) controls for cell viability were fresh cell culture media and 1% (v/v) Triton X-100 solution, respectively. The statistical analysis was carried out using the unpaired Student’s t-test in comparison with the negative control where the significant differences were set at *p<0.05, **p<0.01, and ***p<0.001. Columns represent the mean ± s.d. (n=4).