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. 2021 Jun 2;48(1):144. doi: 10.3892/ijmm.2021.4977

Figure 6.

Figure 6

Beclin-1 contributes to HK-2 cell autophagy by modulating ATG16 expression. (A) HK-2 cells were transfected with ATG16 siRNA, miR-30a-5p mimic, miR-30a-5p inhibitor, or a combination of ATG16 siRNA with miR-30a-5p mimic or miR-30a-5p inhibitor, and then exposed to H/R injury. Protein expression levels were determined by western blot analysis (n=3). (B) Immunofluorescence staining was performed to detect LC3 punctae in HK-2 cells (n=3; magnification, x40). (C) Cell apoptosis was analyzed by flow cytometry following staining for Annexin V-FITC/PI. (n=3). *P<0.05 vs. H/R. #P<0.05 vs. H/R + ATG16 siRNA. ATG16, autophagy-related gene16; H/R, hypoxia/reoxygenation; miR, microRNA; siRNA, small interfering RNA.