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. 2021 May 31;48(1):141. doi: 10.3892/ijmm.2021.4974

Figure 3.

Figure 3

PTEN is the direct target of miR-130b in PC12 and hippocampal cells. (A) Sequence of binding sites between PTEN-3′-UTR and miR-130b. (B) Relative luciferase activities were detected in 293T cells co-transfected with PTEN 3′-UTR (Wt or mut) reporter plasmid, miR-130b mimic or NC mimic. (C) Reverse transcription-quantitative PCR analysis of PTEN mRNA expression in PC12 and hippocampal cells transfected with miR-130b mimic, NC mimic, miR-130b inhibitor and NC inhibitor. U6 served as the reference gene. *P<0.05, **P<0.01. PTEN, phosphatase and tensin homolog; UTR, untranslated region; miR, microRNA; Wt, wild-type; mut, mutant; NC, negative control.