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. 2021 May 26;48(1):138. doi: 10.3892/ijmm.2021.4971

Figure 5.

Figure 5

LBX2-AS1 serves as a sponge of hsa-miR-491-5p in colorectal cancer cells. (A and B) Subcellular locations of LBX2-AS1 were predicted using the lncATLAS and lncLocator databases. (C) Subcellular locations of LBX2-AS1 were determined in HT29 and SW620 colorectal cancer cells. (D) Dual-luciferase reporter assay showed that there was a direct target relationship between hsa-miR-491-5p and LBX2-AS1. **P<0.01 vs. NC. (E) Schematic diagram of the binding site of hsa-miR-491-5p and LBX2-AS1. (F) A weak negative correlation was found between hsa-miR-491-5p and LBX2-AS1, which was identified from the starBase database. (G and H) miR-491-5p expression was decreased in colorectal cancer tissues and cells, as determined via RT-qPCR. **P<0.01 vs. normal tissues or FHC cells. (I) There was a moderate negative correlation between LBX2-AS1 and miR-491-5p in a cohort of 145 patients with colorectal cancer (r=-0.4345, P<0.0001). (J) miR-491-5p expression was determined in colorectal cancer cells transfected with miR-491-5p mimics via RT-qPCR. (K) Dual-luciferase reporter assay confirmed that LBX2-AS1 could act as a sponge of hsa-miR-491-5p in colorectal cancer cells. (L) miR-491-5p expression was determined in colorectal cancer cells transfected with si-LBX2-AS1 via RT-qPCR. (M) The relative LBX2-AS1 expression was quantified in colorectal cancer cells following transfection with miR-491-5p mimics, as determined via RT-qPCR. **P<0.01 vs. miR-NC or si-NC. LBX2-AS1, LBX2 antisense RNA 1; si-, small interfering RNA; NC, negative control; miR, microRNA; RT-qPCR, reverse transcription-quantitative PCR; WT, wild-type; MUT, mutant.