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. Author manuscript; available in PMC: 2021 Jun 3.
Published in final edited form as: Nat Metab. 2021 Apr 12;3(4):513–522. doi: 10.1038/s42255-021-00366-y

Extended Data Fig. 9 |. Colchicine-induced GDF15 inhibits pro-IL-1β expression and mature IL-1β secretion from macrophages.

Extended Data Fig. 9 |

Ex vivo activation of immortalized BMDMs evaluated by the levels of (a–b) pro-IL-1β expression and (d–e) IL-1β secretion. a–b, Cells were pre-incubated with plasma from the indicated mice and then activated with Pam3CSK4. Expression of colchicine-induced GDF15 in hepatocytes was required for plasma activity on blocking pro-IL-1β expression. GalNAc-siLuc, hepatocyte-specific sirNA against luciferase. GalNAc-siGDF15, hepatocyte-specific sirNA against GDF15. c, Activation methods for measuring IL-1β production. d, Colchicine did not alter IL-1β production assayed with two activation methods. 5 Samples per condition over 3 biological replicates e, Colchicine-induced GDF15 was required for plasma activity on reducing IL-1β production. 4 Samples per condition over 3 biological replicates Data are represented as mean ± s.d. Two-sided t-tests were used for statistical analysis.