Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Danio rerio) | ccm2 | http://www.ensembl.org/ | ENSDARG00000013705 | |
| Gene (Danio rerio) | klf2a | http://www.ensembl.org/ | ENSDARG00000042667 | |
| Strain, strain background (Danio rerio) | ccm2m201 | zfin.org | ZDB-ALT-980203–523 | |
| Strain, strain background (Danio rerio) | klf2aig4 | zfin.org | ZDB-ALT-161103–5 | |
| Strain, strain background (Danio rerio) | Tg(fli1:EGFP)y1 | zfin.org | ZDB-ALT-011017–8 | |
| Strain, strain background (Danio rerio) | Tg(gata1:dsred)sd2 | zfin.org | ZDB-ALT-051223–6 | |
| Strain, strain background (Danio rerio) | Tg(klf2a:H2b-EGFP) | zfin.org | ZDB-ALT-161017–10 | |
| Strain, strain background (Danio rerio) | Tg(fli1:negfp)y7 | zfin.org | ZDB-ALT-060821–4 | |
| Strain, strain background (Danio rerio) | Tg(kdrl:mcherry)is5 | zfin.org | ZDB-ALT-110127–25 | |
| Recombinant DNA reagent | pCS2-nls-zCas9-nls | addgene.org | 47929 | |
| Recombinant DNA reagent | pT7-gRNA | addgene.org | 46759 | |
| Commercial assay or kit | mMESSAGE mMACHINE SP6 Transcription Kit | Thermo Fisher Scientific Wlatham, MA |
AM1340 | |
| Commercial assay or kit | MEGAshortscript T7 Transcription kit | Thermo Fisher Scientific, Waltham, MA | AM1333 | |
| Sequence-based reagent | crRNA-1 | This paper | ccm2 gRNA | GGTGTTTCTGAAAGGGGAGA |
| Sequence-based reagent | crRNA-2 | This paper | ccm2 gRNA | GGAGAAGGGTAGGGATAAGA |
| Sequence-based reagent | crRNA-3 | This paper | ccm2 gRNA | GGGTAGGGATAAGAAGGCTC |
| Sequence-based reagent | crRNA-4 | This paper | ccm2 gRNA | GGACAGCTGACCTCAGTTCC |
| Chemical compound, drug | ccm2-MO | zfin.org | ZDB-MRPHLNO-060821–3 | GAAGCTGAGTAATACCTTAACTTCC |
| Chemical compound, drug | tnnt-MO | zfin.org | ZDB-MRPHLNO-060317–4 | CATGTTTGCTCTGATCTGACACGCA |
| Chemical compound, drug | gata1-MO | zfin.org | ZDB-MRPHLNO-050208–10 | CTGCAAGTGTAGTATTGAAGATGTC |
| Chemical compound, drug | tif1γ -MO | afin.org | ZDB-MRPHLNO-110321–1 | GCTCTCCGTACAATCTTGGCCTTTG |
| Chemical compound, drug | klf2a-MO | afin.org | ZDB-MRPHLNO-100610–8 | GGACCTGTCCAGTTCATCCTTCCAC |
| Chemical compound, drug | klf2b-MO | zfin.org | ZDB-MRPHLNO-150427–1 | AAAGGCAAGGTAAAGCCATGTCCAC |
| Software | Volocity | PerkinElmer Waltham, MA |
Volocity | |
| Software | ZEN | Zeiss, Oberkochen, German | ZEN 2.3 SP1 | |
| Software | ImageJ software | ImageJ (http://imagej.nih.gov/ij/) | RRID:SCR_003070 | |
| Software | GraphPad Prism software | GraphPad Prism (https://graphpad.com) | Prism five for Windows | Version 5.01 |