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. 2021 May 14;10:e64943. doi: 10.7554/eLife.64943

Figure 1. Mapping subcellular localizations of the kinome.

(A) Coverage of the kinome (pie graph) and kinase families (bar graph) by the plasmid library. (B) Composition of the kinome library. Species origins (left), epitope tags (middle), and tag positions (right) were summarized. (C) Expression levels of kinase plasmids. HeLa cells were transfected and expression levels were determined by western blotting. (D) Ten cellular compartments with organelle markers (top) and representative kinases (bottom). SEC61B was visualized by GFP tag. TOM20, GM130, Catalase, microtubule, and pericentrin were stained by specific antibodies. F-actin was marked by phalloidin. Other proteins were transfected and stained for epitope tags. (E) Subcellular distribution of the kinome. (F) Kinome tree with localization information. Each dot represents a kinase. Color denotes compartment and size reflects localization score. (G) Family-enriched distributions for kinases. Kinase families were clustered by the ratio of localization to different compartments (Figure 1—figure supplement 1).

Figure 1—source data 1. Raw data for Figure 1 and Figure 1—figure supplement 1.

Figure 1.

Figure 1—figure supplement 1. Mapping subcellular localizations of the kinome.

Figure 1—figure supplement 1.

(A) Distribution of the kinome plasmid library on the kinome tree. Each red dot represents a kinase in the library. (B) Ratio of N-terminal- and C-terminal-tagged kinases in each kinase family. (C) Flowchart of kinome subcellular localization mapping. (D) Distribution of 10 subcellular compartments in each kinase family (left panel) and in kinases with different tag positions (right panel).