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A schematic illustration is shown for the application of MHC multimer technology, including cell enrichment or depletion (A), combinatorial detection in a single sample (B), antigen-specific T-cell stimulation and amplification using artificial antigen-presenting complex (C), and multi-parametric detection of rare T cells using mass cytometry with isotope-labeled MHC multimers or DNA barcoded MHC multimers (D).
