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. 2021 May 6;40(22):3799–3814. doi: 10.1038/s41388-021-01783-9

Fig. 5. GLI1 overexpression rescues the effects of combined inhibition of SMO and BRD4.

Fig. 5

A Western blot of BRD4 and GLI1 in melanoma cells transduced with LV-c or LV-GLI1 after treatment with MRT-92, MZ1 or their combination for 72 h. HSP90 was used as loading control. B Cell viability of SSM2c, A375 and MeWo cells transduced with LV-c or LV-GLI1 after 72 h of treatment with MRT-92, MZ1 or combination at the following concentrations: SSM2c: MRT-92 at 250 nM and MZ1 at 125 nM; A375: MRT-92 at 250 nM and MZ1 at 250 nM; MeWo: MRT-92 at 300 nM and MZ1 at 125 nM. Data are expressed as fold change of vehicle (DMSO) ± SEM. (n = 3). P values were calculated by one-way ANOVA with Tukey’s test. *, p < 0.05; **, p < 0.01.