Fig. 4. Protein synthesis inhibition by PERK activity during GR promotes apoptosis.

A Time lapse of ³⁵S-methionine incorporation to new polypeptides during GD/GR in cortical neurons. A representative autoradiograph of ³⁵S-methionine incorporation to new polypeptides resolved by SDS-PAGE. B Effect of PERK inhibition during GR on protein synthesis in cortical neurons. A representative autoradiograph of ³⁵S-methionine incorporation to new polypeptides in neurons treated with PERK inhibitor (GSK, 10 µM), drug treatment was only during GR. C Quantification and representative micrographs of TUNEL-positive cells exposed to 2 h of GD and 16 h of GR in the presence or absence of PERK inhibitor (GSK, 10 µM). The drug was administered only in the GR phase. Data represent the mean ± SEM of three independent experiments and were analyzed by one-way ANOVA followed by Fisher’s multiple comparison test, *p < 0.05 vs. control, ^&p < 0.05 vs. 2 h GD + 16 h GR. Scale bar = 20 µm.