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. 2021 Jun 3;7:131. doi: 10.1038/s41420-021-00518-9

Fig. 5. Downregulation of 14-3-3 θ mRNA by IRE1α RIDD activity stimulates ASK1 activation.

Fig. 5

A Comparison of human and rat 14-3-3 θ mRNA XBP1 like consensus site (gray). B Predicted secondary structure of the 14-3-3 θ mRNA folded in silico by the RNAfold web server. XBP1 like consensus site (gray area). C Effect of the inhibition of IRE1 RNase activity in the expression of 14-3-3 θ mRNA at different times of GD and GR. STF inhibitor (30 µM) was incubated during GD (2 h) or GR (4, 8, and 16 h). D Representative immunoblot and quantification of 14-3-3 θ /β-Actin at different times of GD and GR. E Representative immunoblot of 14-3-3 θ /β-Actin the presence or the absence of STF (30 µM) incubated during GR. F Analysis of the interaction of 14-3-3 θ with ASK1 by co-immunoprecipitation and detected by immunoblot in neurons exposed to GD and GR. G Representative co-immunoprecipitation of 14-3-3 θ and ASK1 in the presence or the absence of STF (30 µM) incubated during GR. Data represent the mean ± SEM of three independent experiments and were analyzed by unpaired Student’s t test (C) and one-way ANOVA followed by Fisher’s multiple comparison test (D), *p < 0.05 vs. control.