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. 2021 Jun 3;7:131. doi: 10.1038/s41420-021-00518-9

Fig. 6. ASK1–JNK contributes to GD/GR-induced neuronal death.

Fig. 6

A Representative immunoblot and quantification of p-ASK1 at different times of GD and GR. B Subcellular localization of JNK in cortical neurons exposed to GD/GR. Representative immunoblot and quantifications of JNK (46 kDa isoform) in the nucleus and cytoplasm. C Chop mRNA levels in cortical neurons treated with the JNK inhibitor (SP, 10 µM) or ASK1 inhibitor (MSC, 1 µM). D MTT Reduction and E LDH activity in cortical neurons exposed to GD/GR, in the presence or absence of the ASK1 (MSC, 1 µM) or the JNK inhibitors (SP, 10 µM) during GR. Data represent the mean ± SEM of three independent experiments and were analyzed by one way ANOVA followed by Fisher’s multiple comparison test, *p < 0.05 vs. control, &p < 0.05 vs. 2 h GD + 16 h GR (C, E) or vs. 2 h GD + 22 h GR (D).