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. 2021 Jun 3;7:131. doi: 10.1038/s41420-021-00518-9

Fig. 7. IRE1α RNase activity regulates the ASK1–JNK pathway and induces apoptotic neuronal damage.

Fig. 7

A Subcellular localization of JNK in the presence or absence of the IRE1 RNase inhibitor (STF, 30 µM) incubated during GR. Representative immunoblot and quantifications of JNK in the nucleus and cytoplasm. B Chop expression in cortical neurons exposed to GD/GR in the presence or absence of IRE1 RNase inhibitor (STF, 30 µM) incubated during GR. C Quantification and representative micrographs of TUNEL-positive cells exposed to 2 h of GD and 16 h of GR in the presence or absence of IRE1 RNase (STF, 30 µM), ASK1 (MSC, 1 µM), and JNK (SP, 10 µM) inhibitors. The drugs were administered only in the GR phase. Data represent the mean ± SEM of 3 (A, B) and 4 (C) independent experiments and were analyzed by one way ANOVA followed by Fisher’s multiple comparison test, *p < 0.05 vs. control, &p < 0.05 vs. 2 h GD + 16 h GR. Scale bar = 20 µm.