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. 2021 Jun 3;4:674. doi: 10.1038/s42003-021-02199-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a O6-mFc detects galactose-3-sulfate on the N-glycans of bovine thyroglobulin and O-glycans on human mucins. b O6-mFc bound to sulfatide and not porcine gangliosides in TLC-overlay. c Validation of b and c by ELISA, using O6-mFc at 4 concentrations. n = 2, each black triangle represents one data point, error bars = ±1 standard deviation. d Dose-dependent binding is observed by flow cytometry of Pro-5 CHO cells and not Lec8 mutants. Lec8GT CHO cells are positive; however, addition of α1-3 linked fucose to the LDN motif abrogates binding in Lec8GTFT cells.