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. 2021 Jun 3;11:11766. doi: 10.1038/s41598-021-90154-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Differential expression of individually validated miRs from IS + and SS + (unadjusted data). The highest ranking 5 cardiovascular disease (CVD)-related EC-EV miRs initially identified in pooled RNA sample sets from IS and SS were validated by qPCR in individual RNA samples. IS + : Interrupted Sitter group with enhanced ethnic diversity (N = 25); SS + : Super Sitter group with enhanced ethnic diversity (N = 60). Statistical significance between IS + and SS + groups was examined by unadjusted t-test. Horizonal bars indicate group means.