Fig. 1.

Tas2r138 increased after P. aeruginosa infection. AMs and neutrophils were isolated from C57BL/6N mice and infected with PAO1 (MOI: 5:1) for 1 or 2 h. Total RNA was extracted for reverse transcription and qPCR. a–d The Tas2r family mRNAs were expressed as the fold difference to GAPDH in neutrophils (a) and AMs (b), and the protein expressions in neutrophils and AMs were exhibited by confocal microscopy imaging (c, d) (arrows showing the increased TAS2R138). e, f AMs and neutrophils were infected with AHL-12 (50 µM) for 2 h. The Tas2r138 mRNAs were expressed as the fold difference to GAPDH in neutrophils and AMs. g, h The G-protein family mRNAs were expressed as the fold difference to GAPDH in neutrophils and AMs. The results were expressed as the mean ± SD and the significant difference level between two groups was defined by one-way ANOVA with Tukey post hoc tests, *p < 0.05 (control short as CT in all figures)