Fig. 6.

Tas2r138 played protective roles against P. aeruginosa infection in vivo. a Eight to 12 weeks male and female (sex-matched) mice were subjected to neutrophil depletion by injecting (i.p.) 0.1 mg GR-1 monoclonal antibody with rat IgG as control at 24 or 48 h before bacterial infection. AMs were depleted by i.n. instillation of 100 µl of clodronate-containing 48 h before infection with 1 × 10⁷ PAO1.⁴⁴ Mouse survival was monitored for 7 days. Survival was represented by Kaplan–Meier curves. (p = 0.0273, n = 6, log-rank test). b, c After AMs and neutrophils depletion, 1 × 10⁶ normal or siTas2r138 AMs or siTas2r138 neutrophils were transferred by I.V. injection. d, e CFU and total cells test in BAL (B blocking, D depletion), MPO test, cytokines test (from serum), and histochemical staining were performed after 24 h post infection and euthanasia mice (blue arrows showing the 10× augmented view for indicated areas). The results were expressed as the mean ± SD and the significant difference level between two groups was defined by student t tests, *p < 0.05. AM− AM cell deletion, AM+ AM cell deletion and then transferred AM back, AM + si AM cell deletion and then transferred siTas2r138 AM back, Neu− Neu blocking, Neu+ Neu blocking and then transferred Neu back, Neu + si Neu blocking and then transferred siTas2r138 Neu back