Table 2.

PERMANOVA results for total beta diversity (SOR), the turnover component of beta diversity (SIM), and the nested component of beta diversity (SNE).

	Df	Sums of squares	Mean squares	F test	R2	p value
Total beta diversity
P. destructans status	1	0.746	0.746	1.7403	0.006	0.018*
Ecoregion	2	1.427	0.713	1.664	0.012	0.011*
year	1	0.795	0.794	1.8541	0.007	0.001***
site	45	24.357	0.541	1.2626	0.216	0.771
P. destructans status:Ecoregion	2	0.905	0.452	1.055	0.008	0.107
Residuals	197	84.454	0.428		0.749
Total	248	112.683			1
Turnover
P. destructans status	1	0.862	0.861	2.139	0.008	0.003**
Ecoregion	2	1.591	0.795	1.975	0.014	0.033*
year	1	0.605	0.605	1.502	0.005	0.012*
site	45	23.712	0.526	1.308	0.221	0.72
P. destructans status:Ecoregion	2	0.876	0.437	1.087	0.008	0.22
Residuals	197	79.344	0.402		0.741
Total	248	106.99			1
Nestedness
P. destructans status	1	− 0.007	− 0.007	− 7.92	− 0.045	1
Ecoregion	2	− 0.01	− 0.005	− 5.955	− 0.067	0.347
year	1	0.009	0.009	11.111	0.063	0.105
site	45	− 0.01	− 0.0002	− 0.269	− 0.069	0.737
P. destructans status:Ecoregion	2	− 0.0003	− 0.0001	− 0.22	− 0.002	0.745
Residuals	197	0.174	0.0008		1.121
Total	248	0.156			1

There is a significant difference between P. destructans status, Ecoregion, and year for both SOR and SIM (p < 0.05).