Fig. 4.

Intestinal fat absorption in Sar1b^mut/+ and Sar1b^del/+ mice. Wild-type, Sar1b^mut/+, and Sar1b^del/+ female and male mice (9–11 weeks) were fed ad libitum with a conventional chow diet for 1 week. Prior to the sacrifice, mice were fasted 6 h and then were given 200 μl of olive oil and 4 μCi [¹⁴C]-triolein by oral gavage. Ten minutes later, mice were peritoneally injected with 1 mg/kg of Pluronic F-127 to inhibit lipoprotein lipase-induced chylomicron catabolism. Blood was taken at 60, 90, and 120 min time points. Plasma (A, B) triglycerides and (C, D) radioactivity derived from the labeled [¹⁴C]-triolein were determined as described in the Materials and Method section. At 120 min, chylomicrons (CM) content was determined by ultracentrifugation. E: Triglycerides, (F) total cholesterol, and (G) CM scintillation counts were analyzed in this fraction. Finally, feces were dried and (H) triglycerides were measured as described previously. Results represent the means ± SEM of 10–13 mice in each group. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗∗P < 0.0001 versus wild-type mice (Ctrl).