Fig. 7.

Impact of Sar1b genetic defects on intestinal and liver lipid metabolism. Wild-type, Sar1b^mut/+, and Sar1b^del/+ female and male mice (9–11 weeks) were fed ad libitum with a conventional chow diet for 1 week. Prior to the sacrifice, mice were fasted 6 h and then were given 200 μl of olive oil and 4 μCi [¹⁴C]-triolein by oral gavage. Protein expression of important markers of (A, B) fatty acid β-oxidation (ACADL, CPT1a, PGC1α, and PPARα) and (D, E) lipogenesis (ACC, P-ACC, AMPKα, and P-AMPKα) were analyzed in the intestine and liver by Western blot as described in the Materials and Method section. The gene expression was also assessed in (C) intestine and (F) liver by RT-qPCR as described in Materials and Method section. Results represent the means ± SEM of 3–6 mice in each group. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 versus wild-type mice (Ctrl).