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. 2021 Jun 3;11:11695. doi: 10.1038/s41598-021-91203-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Culture of iPSC motor neuron spheroids within 3D collagen I matrices enhances mRNA expression of heavy neurofilament (SMI-32), vesicular transporter of acetylcholine (VAChT) and axonal extension (MAPT). (a) Confocal tile scans of motor neuron progenitor spheroids suspended within 3D collagen I matrices of different concentrations (0.5, 1 and 1.7 mg/mL) adhere and extend axons over 5 days. (b) mRNA expression of mature motor neuron markers SMI32 and (c) VACHT, and (d) MAPT as indication of amount of axonal extension compared to adhered monolayer spheroids. Data presented ± standard deviation (SD). *P ≤ 0.05 and ***P ≤ 0.001. Scale bars = 200 µm.