Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jun 3;4:672. doi: 10.1038/s42003-021-02225-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — a ELISA of secreted IL-2 in the supernatants of Jurkat cells collected 24 h following stimulation by magnetic beads. Jurkat T cells either expressed a non-targeting shRNA (control) or PAG targeting shRNA (shPAG). For rescue transfections, shPAG Jurkats were transiently transfected with full length, wild type PAG (PAG FL) or with phosphodeficient mutants (Y181A, Y317A, and Y417A). b Adhesion assay of Jurkat cells to fibronectin following stimulation for 15 min. The number of adherent cells remaining, expressed as a percentage of the total number of labeled cells, was determined with a fluorescent plate reader. PAG knockdown and rescue transfections as in a. c Percentage of Jurkat cells expressing CD69 on the surface following 24-hour stimulation, measured by flow cytometry. PAG knockdown and rescue transfections as in a. d, e Phosphorylated ERK (d) and phosphorylated SRC (e) were detected by western blot of Jurkat lysates 5 min after stimulation by magnetic beads. PAG knockdown and rescue transfections as in a. Fold change is calculated relative to anti-CD3 stimulation (d) or unstimulated (e). Individual data points shown with median indicated of three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns not significant.