Fig. 6. In the absence of PAG, T cells are more active.

a Immunohistochemical staining for CD3⁺, CD4⁺, CD8⁺, and granzyme B⁺ (GZMB) infiltrating cells in MC38 tumors from WT and PAG KO mice at day 19 after tumor implantation. b Quantification of CD3⁺, CD4⁺, CD8⁺, and GZMB⁺ cells per high powered field (HPF) of the staining represented in a. Ten to fifteen HPFs were analyzed from each group. c IL-2 primed splenocytes (effector cells) from WT and PAG KO mice were co-cultured overnight with L929 cells (target cells). The release of LDH from the lysed target cells was measured and cytotoxicity was calculated relative to target cells cultured in the absence of primed T cells. d Purified CD8⁺ T cells (effector cells) were isolated from WT and PAG KO mice and co-cultured with Raji cells (target cells) pre-loaded with SEE. The release of LDH from the lysed target cells was measured and % cytotoxicity was calculated relative to target cells cultured in the absence of primed T cells. *p < 0.05; ***p < 0.001; ****p < 0.0001.